Ultima Genomics›Ultima Genomics› offers ppmSeq

Ultima says ppmSeq uses information from both strands of the same DNA molecule to remove false-positive single-nucleotide calls caused by sample damage. Its product page reports sensitivity for rare events and describes a PCR-free adapter workflow.

The approach is designed for low-frequency variants in cell-free DNA and residual-disease research. The performance figures are the manufacturer’s stated results and need matched samples and assay settings for comparison with other panels.

A July 2024 Labcorp announcement explicitly named Ultima’s UG 100 solution and ppmSeq technology in an expanded collaboration. It described research into whole-genome sequencing and oncology testing applications.

That dated announcement establishes ppmSeq as an offered technology by 2024. Ultima’s current product page describes the paired-strand method and its associated adapters and protocols; it does not establish a more precise first-release date.

The ppmSeq method combines Ultima’s flow chemistry with emulsion-based clonal amplification and strand concordance. Ultima states that its adapters retain information from both DNA strands without relying on unique molecular identifiers.

The published protocol table separates genomic-DNA and cell-free-DNA inputs. The method depends on the UG 100 Solaris Free workflow, so its rare-variant application should remain distinct from the sequencer and the broader Solaris preparation family.

ppmSeq is Ultima Genomics’ paired plus-minus sequencing method for rare single-nucleotide variant detection. The official page describes capturing information from complementary strands of a DNA template to distinguish true variants from sample artifacts.

The workflow uses dedicated adapters with Solaris Free preparation and the UG 100 platform. Ultima presents genomic-DNA and cell-free-DNA protocols and describes research applications that include liquid biopsy and minimal residual disease.