412 Venture Fund focuses on early-stage investments in startups, primarily in the Pittsburgh region. The fund targets companies in sectors such as autonomy, robotics, AI, advanced materials, and healthcare IT. Their investment strategy spans pre-seed, seed, and Series A rounds, supporting companies through various stages of growth.

As former entrepreneurs and operators, the fund's partners bring valuable experience to their portfolio companies. They offer support beyond capital, including assistance with achieving funding milestones, connecting to major customers, finding experts, making key hires, and positioning for successful exits. Notable portfolio companies include Edge Case Research, Gridwise, and Vali Cyber.